Human Nuclear Receptor 4A1 (NR4A1;NUR77) ELISA Kit

Cat.#:

JL19195

Regulatory Status:

RUO

Alternative:

NR4A1; GFRP1; HMR; N10; NAK-1; NGFIB; NP10; NUR77; TR3; nuclear receptor subfamily 4 group A member 1; NH41

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.074ng/mL

Specificity:

This kit recognizes Human NR4A1;NUR77 in samples. No significant cross-reactivity or interference between Human NR4A1;NUR77 and analogues was observed.

Sample Type:

Tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Neuroscience

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| NR4A1;NUR77

The nuclear receptor 4A1 (NR4A1 for "nuclear receptor subfamily 4 group A member 1") also known as Nur77, TR3, and NGFI-B is a protein that is encoded by the NR4A1 gene.Nuclear receptor 4A1 (NR4A1) is a member of the NR4A nuclear receptor family of intracellular transcription factors.NR4A1 is involved in cell cycle mediation, inflammation and apoptosis.Nuclear receptor 4A1 plays a key role in mediating inflammatory responses in macrophages.In addition, subcellular localization of the NR4A1 protein appears to play a key role in the survival and death of cells.Expression is inducible by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. Translocation of the protein from the nucleus to mitochondria induces apoptosis.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.22	1.56	0.92	0.63	0.42	0.25	0.18	0.08
Corrected OD	2.14	1.48	0.84	0.55	0.34	0.17	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human NR4A1;NUR77 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-106	103
Cell Culture Supernates(n=8)	96-108	104

| Linearity

Samples were spiked with high concentrations of Human NR4A1;NUR77 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-102	87-108	105-114
1 : 4	Average (%)	94	98	107